AustralieFrV1, Main, Exploration, bibRecord, 001464

Validation of 14-3-3 Protein as a Marker in Sporadic Creutzfeldt-Jakob Disease Diagnostic.

Identifieur interne : 001464 ( Main/Exploration ); précédent : 001463; suivant : 001465

Validation of 14-3-3 Protein as a Marker in Sporadic Creutzfeldt-Jakob Disease Diagnostic.

Auteurs : Matthias Schmitz [Allemagne] ; Elisabeth Ebert [Allemagne] ; Katharina Stoeck [Allemagne] ; André Karch [Allemagne] ; Steven Collins [Australie] ; Miguel Calero [Espagne] ; Theodor Sklaviadis [Grèce] ; Jean-Louis Laplanche [France] ; Ewa Golanska [Pologne] ; Ines Baldeiras [Portugal] ; Katsuya Satoh [Japon] ; Raquel Sanchez-Valle [Espagne] ; Anna Ladogana [Italie] ; Anders Skinningsrud [Norvège] ; Anna-Lena Hammarin [Suède] ; Eva Mitrova [Slovaquie] ; Franc Llorens [Allemagne] ; Yong Sun Kim [Corée du Sud] ; Alison Green [Royaume-Uni] ; Inga Zerr [Allemagne]

Source :

Molecular neurobiology [ 1559-1182 ] ; 2016.

RBID : pubmed:25947081

Descripteurs français

English descriptors

KwdEn :
- 14-3-3 Proteins (cerebrospinal fluid), 14-3-3 Proteins (metabolism), Biomarkers (metabolism), Blotting, Western, Creutzfeldt-Jakob Syndrome (cerebrospinal fluid), Creutzfeldt-Jakob Syndrome (diagnosis), Creutzfeldt-Jakob Syndrome (metabolism), Enzyme-Linked Immunosorbent Assay, Humans, Laboratories, Preservation, Biological, Protein Isoforms (metabolism), Reference Standards, Reproducibility of Results, S100 Proteins (metabolism), Sensitivity and Specificity, tau Proteins (metabolism).
MESH :
- chemical , cerebrospinal fluid : 14-3-3 Proteins.
- chemical , metabolism : 14-3-3 Proteins, Biomarkers, Protein Isoforms, S100 Proteins, tau Proteins.
- cerebrospinal fluid : Creutzfeldt-Jakob Syndrome.
- diagnosis : Creutzfeldt-Jakob Syndrome.
- metabolism : Creutzfeldt-Jakob Syndrome.
- Blotting, Western, Enzyme-Linked Immunosorbent Assay, Humans, Laboratories, Preservation, Biological, Reference Standards, Reproducibility of Results, Sensitivity and Specificity.

Abstract

At present, the testing of 14-3-3 protein in cerebrospinal fluid (CSF) is a standard biomarker test in suspected sporadic Creutzfeldt-Jakob disease (sCJD) diagnosis. Increasing 14-3-3 test referrals in CJD reference laboratories in the last years have led to an urgent need to improve established 14-3-3 test methods. The main result of our study was the validation of a commercially available 14-3-3 ELISA next to the commonly used Western blot method as a high-throughput screening test. Hereby, 14-3-3 protein expression was quantitatively analyzed in CSF of 231 sCJD and 2035 control patients. We obtained excellent sensitivity/specificity values of 88 and 96% that are comparable to the established Western blot method. Since standard protocols and preanalytical sample handling have become more important in routine diagnostic, we investigated in a further step the reproducibility and stability of 14-3-3 as a biomarker for human prion diseases. Ring trial data from 2009 to 2013 revealed an increase of Fleiss' kappa from 0.51 to 0.68 indicating an improving reliability of 14-3-3 protein detection. The stability of 14-3-3 protein under short-term and long-term storage conditions at various temperatures and after repeated freezing/thawing cycles was confirmed. Contamination of CSF samples with blood appears likely to be an important factor at a concentration of more than 2500 erythrocytes/μL. Hemolysis of erythrocytes with significant release of 14-3-3 protein started after 2 days at room temperature. We first define clear standards for the sample handling, short- and long-term storage of CSF samples as well as the handling of blood- contaminated samples which may result in artificially elevated CSF levels of 14-3-3.

DOI: 10.1007/s12035-015-9167-5
PubMed: 25947081

Affiliations:

Allemagne, Australie, Corée du Sud, Espagne, France, Grèce, Italie, Japon, Norvège, Pologne, Portugal, Royaume-Uni, Slovaquie, Suède
Basse-Saxe, Catalogne, Communauté de Madrid, Latium, Victoria (État)
Barcelone, Göttingen, Madrid, Melbourne, Rome
Université de Melbourne

Le document en format XML

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<author><name sortKey="Sklaviadis, Theodor" sort="Sklaviadis, Theodor" uniqKey="Sklaviadis T" first="Theodor" last="Sklaviadis">Theodor Sklaviadis</name>
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<author><name sortKey="Laplanche, Jean Louis" sort="Laplanche, Jean Louis" uniqKey="Laplanche J" first="Jean-Louis" last="Laplanche">Jean-Louis Laplanche</name>
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</affiliation>
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<author><name sortKey="Satoh, Katsuya" sort="Satoh, Katsuya" uniqKey="Satoh K" first="Katsuya" last="Satoh">Katsuya Satoh</name>
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</affiliation>
</author>
<author><name sortKey="Sanchez Valle, Raquel" sort="Sanchez Valle, Raquel" uniqKey="Sanchez Valle R" first="Raquel" last="Sanchez-Valle">Raquel Sanchez-Valle</name>
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<author><name sortKey="Ladogana, Anna" sort="Ladogana, Anna" uniqKey="Ladogana A" first="Anna" last="Ladogana">Anna Ladogana</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
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</placeName>
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<author><name sortKey="Skinningsrud, Anders" sort="Skinningsrud, Anders" uniqKey="Skinningsrud A" first="Anders" last="Skinningsrud">Anders Skinningsrud</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Multidisiplinary Laboratory Medicine and Medical Biochemistry Division of Diagnostics and Technology Akerhus University Hospital 1478 Lørenskog, 1478, Lørenskog, Norway.</nlm:affiliation>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>Department of Multidisiplinary Laboratory Medicine and Medical Biochemistry Division of Diagnostics and Technology Akerhus University Hospital 1478 Lørenskog, 1478, Lørenskog</wicri:regionArea>
<wicri:noRegion>Lørenskog</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hammarin, Anna Lena" sort="Hammarin, Anna Lena" uniqKey="Hammarin A" first="Anna-Lena" last="Hammarin">Anna-Lena Hammarin</name>
<affiliation wicri:level="1"><nlm:affiliation>The Public Health Agency of Sweden, Unit for highly pathogenic viruses, 171 82, Solna, Sweden.</nlm:affiliation>
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<author><name sortKey="Mitrova, Eva" sort="Mitrova, Eva" uniqKey="Mitrova E" first="Eva" last="Mitrova">Eva Mitrova</name>
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<country xml:lang="fr">Slovaquie</country>
<wicri:regionArea>Department of Prion Diseases, Slovak Medical University Bratislava, Limbová 14, 833-03, Bratislava</wicri:regionArea>
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<author><name sortKey="Llorens, Franc" sort="Llorens, Franc" uniqKey="Llorens F" first="Franc" last="Llorens">Franc Llorens</name>
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<country xml:lang="fr">Allemagne</country>
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<author><name sortKey="Kim, Yong Sun" sort="Kim, Yong Sun" uniqKey="Kim Y" first="Yong Sun" last="Kim">Yong Sun Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Ilsong Institute of Life Science, College of Medicine, Hallym University, Anyang, Republic of Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
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</affiliation>
</author>
<author><name sortKey="Green, Alison" sort="Green, Alison" uniqKey="Green A" first="Alison" last="Green">Alison Green</name>
<affiliation wicri:level="1"><nlm:affiliation>Western General Hospital, National Creutzfeldt-Jakob Disease Surveillance Unit, Crewe Road, Edinburgh, EH4 2XU, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Western General Hospital, National Creutzfeldt-Jakob Disease Surveillance Unit, Crewe Road, Edinburgh, EH4 2XU</wicri:regionArea>
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</affiliation>
</author>
<author><name sortKey="Zerr, Inga" sort="Zerr, Inga" uniqKey="Zerr I" first="Inga" last="Zerr">Inga Zerr</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Neurology, University Medical Center Göttingen and German Center for Neurodegenerative Diseases (DZNE)-site Göttingen, Robert-Koch Str. 40, 37075, Göttingen, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Neurology, University Medical Center Göttingen and German Center for Neurodegenerative Diseases (DZNE)-site Göttingen, Robert-Koch Str. 40, 37075, Göttingen</wicri:regionArea>
<placeName><region type="land" nuts="2">Basse-Saxe</region>
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<author><name sortKey="Ebert, Elisabeth" sort="Ebert, Elisabeth" uniqKey="Ebert E" first="Elisabeth" last="Ebert">Elisabeth Ebert</name>
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<country xml:lang="fr">Allemagne</country>
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<author><name sortKey="Stoeck, Katharina" sort="Stoeck, Katharina" uniqKey="Stoeck K" first="Katharina" last="Stoeck">Katharina Stoeck</name>
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<country xml:lang="fr">Allemagne</country>
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<author><name sortKey="Karch, Andre" sort="Karch, Andre" uniqKey="Karch A" first="André" last="Karch">André Karch</name>
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<country xml:lang="fr">Allemagne</country>
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<author><name sortKey="Collins, Steven" sort="Collins, Steven" uniqKey="Collins S" first="Steven" last="Collins">Steven Collins</name>
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<country xml:lang="fr">Australie</country>
<wicri:regionArea>Department of Pathology, The University of Melbourne, Parkville, 3010</wicri:regionArea>
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<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
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<author><name sortKey="Calero, Miguel" sort="Calero, Miguel" uniqKey="Calero M" first="Miguel" last="Calero">Miguel Calero</name>
<affiliation wicri:level="3"><nlm:affiliation>Chronic Disease Programme, CIBERNED, and CIEN Foundation-Queen Sofia Foundation, Instituto de Salud Carlos III, Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Chronic Disease Programme, CIBERNED, and CIEN Foundation-Queen Sofia Foundation, Instituto de Salud Carlos III, Madrid</wicri:regionArea>
<placeName><settlement type="city">Madrid</settlement>
<region nuts="2" type="region">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sklaviadis, Theodor" sort="Sklaviadis, Theodor" uniqKey="Sklaviadis T" first="Theodor" last="Sklaviadis">Theodor Sklaviadis</name>
<affiliation wicri:level="1"><nlm:affiliation>Pharmaceutical Sciences, Laboratory of Pharmacology, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece.</nlm:affiliation>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>Pharmaceutical Sciences, Laboratory of Pharmacology, Aristotle University of Thessaloniki, Thessaloniki, 54124</wicri:regionArea>
<wicri:noRegion>54124</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Laplanche, Jean Louis" sort="Laplanche, Jean Louis" uniqKey="Laplanche J" first="Jean-Louis" last="Laplanche">Jean-Louis Laplanche</name>
<affiliation wicri:level="1"><nlm:affiliation>Service de Biochimie et Biologie Moléculaire, Hôpital Lariboisière, Assistance Publique - Hôpitaux de Paris, 2, rue A Paré, 75475, Paris cedex 10, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Service de Biochimie et Biologie Moléculaire, Hôpital Lariboisière, Assistance Publique - Hôpitaux de Paris, 2, rue A Paré, 75475, Paris cedex 10</wicri:regionArea>
<wicri:noRegion>75475, Paris cedex 10</wicri:noRegion>
<wicri:noRegion>75475, Paris cedex 10</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Golanska, Ewa" sort="Golanska, Ewa" uniqKey="Golanska E" first="Ewa" last="Golanska">Ewa Golanska</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Pathology and Neuropathology, Medical University of Lodz, 8/10 Czechoslowacka Str., 92-216, Lodz, Poland.</nlm:affiliation>
<country xml:lang="fr">Pologne</country>
<wicri:regionArea>Department of Molecular Pathology and Neuropathology, Medical University of Lodz, 8/10 Czechoslowacka Str., 92-216, Lodz</wicri:regionArea>
<wicri:noRegion>Lodz</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Baldeiras, Ines" sort="Baldeiras, Ines" uniqKey="Baldeiras I" first="Ines" last="Baldeiras">Ines Baldeiras</name>
<affiliation wicri:level="1"><nlm:affiliation>Center for Neuroscience and Cell Biology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.</nlm:affiliation>
<country xml:lang="fr">Portugal</country>
<wicri:regionArea>Center for Neuroscience and Cell Biology, Faculty of Medicine, University of Coimbra, Coimbra</wicri:regionArea>
<wicri:noRegion>Coimbra</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Satoh, Katsuya" sort="Satoh, Katsuya" uniqKey="Satoh K" first="Katsuya" last="Satoh">Katsuya Satoh</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-12-4, Nagasaki, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-12-4, Nagasaki</wicri:regionArea>
<wicri:noRegion>Nagasaki</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sanchez Valle, Raquel" sort="Sanchez Valle, Raquel" uniqKey="Sanchez Valle R" first="Raquel" last="Sanchez-Valle">Raquel Sanchez-Valle</name>
<affiliation wicri:level="3"><nlm:affiliation>Neurology Department, Hospital Clinic, IDIBAPS, Barcelona, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Neurology Department, Hospital Clinic, IDIBAPS, Barcelona</wicri:regionArea>
<placeName><settlement type="city">Barcelone</settlement>
<region nuts="2" type="region">Catalogne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ladogana, Anna" sort="Ladogana, Anna" uniqKey="Ladogana A" first="Anna" last="Ladogana">Anna Ladogana</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy.</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome</wicri:regionArea>
<placeName><settlement type="city">Rome</settlement>
<region nuts="2">Latium</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Skinningsrud, Anders" sort="Skinningsrud, Anders" uniqKey="Skinningsrud A" first="Anders" last="Skinningsrud">Anders Skinningsrud</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Multidisiplinary Laboratory Medicine and Medical Biochemistry Division of Diagnostics and Technology Akerhus University Hospital 1478 Lørenskog, 1478, Lørenskog, Norway.</nlm:affiliation>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>Department of Multidisiplinary Laboratory Medicine and Medical Biochemistry Division of Diagnostics and Technology Akerhus University Hospital 1478 Lørenskog, 1478, Lørenskog</wicri:regionArea>
<wicri:noRegion>Lørenskog</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hammarin, Anna Lena" sort="Hammarin, Anna Lena" uniqKey="Hammarin A" first="Anna-Lena" last="Hammarin">Anna-Lena Hammarin</name>
<affiliation wicri:level="1"><nlm:affiliation>The Public Health Agency of Sweden, Unit for highly pathogenic viruses, 171 82, Solna, Sweden.</nlm:affiliation>
<country xml:lang="fr">Suède</country>
<wicri:regionArea>The Public Health Agency of Sweden, Unit for highly pathogenic viruses, 171 82, Solna</wicri:regionArea>
<wicri:noRegion>Solna</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mitrova, Eva" sort="Mitrova, Eva" uniqKey="Mitrova E" first="Eva" last="Mitrova">Eva Mitrova</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Prion Diseases, Slovak Medical University Bratislava, Limbová 14, 833-03, Bratislava, Slovakia.</nlm:affiliation>
<country xml:lang="fr">Slovaquie</country>
<wicri:regionArea>Department of Prion Diseases, Slovak Medical University Bratislava, Limbová 14, 833-03, Bratislava</wicri:regionArea>
<wicri:noRegion>Bratislava</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Llorens, Franc" sort="Llorens, Franc" uniqKey="Llorens F" first="Franc" last="Llorens">Franc Llorens</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Neurology, University Medical Center Göttingen and German Center for Neurodegenerative Diseases (DZNE)-site Göttingen, Robert-Koch Str. 40, 37075, Göttingen, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Neurology, University Medical Center Göttingen and German Center for Neurodegenerative Diseases (DZNE)-site Göttingen, Robert-Koch Str. 40, 37075, Göttingen</wicri:regionArea>
<placeName><region type="land" nuts="2">Basse-Saxe</region>
<settlement type="city">Göttingen</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Yong Sun" sort="Kim, Yong Sun" uniqKey="Kim Y" first="Yong Sun" last="Kim">Yong Sun Kim</name>
<affiliation wicri:level="1"><nlm:affiliation>Ilsong Institute of Life Science, College of Medicine, Hallym University, Anyang, Republic of Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Ilsong Institute of Life Science, College of Medicine, Hallym University, Anyang</wicri:regionArea>
<wicri:noRegion>Anyang</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Green, Alison" sort="Green, Alison" uniqKey="Green A" first="Alison" last="Green">Alison Green</name>
<affiliation wicri:level="1"><nlm:affiliation>Western General Hospital, National Creutzfeldt-Jakob Disease Surveillance Unit, Crewe Road, Edinburgh, EH4 2XU, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Western General Hospital, National Creutzfeldt-Jakob Disease Surveillance Unit, Crewe Road, Edinburgh, EH4 2XU</wicri:regionArea>
<wicri:noRegion>EH4 2XU</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zerr, Inga" sort="Zerr, Inga" uniqKey="Zerr I" first="Inga" last="Zerr">Inga Zerr</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Neurology, University Medical Center Göttingen and German Center for Neurodegenerative Diseases (DZNE)-site Göttingen, Robert-Koch Str. 40, 37075, Göttingen, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Neurology, University Medical Center Göttingen and German Center for Neurodegenerative Diseases (DZNE)-site Göttingen, Robert-Koch Str. 40, 37075, Göttingen</wicri:regionArea>
<placeName><region type="land" nuts="2">Basse-Saxe</region>
<settlement type="city">Göttingen</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Molecular neurobiology</title>
<idno type="eISSN">1559-1182</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>14-3-3 Proteins (cerebrospinal fluid)</term>
<term>14-3-3 Proteins (metabolism)</term>
<term>Biomarkers (metabolism)</term>
<term>Blotting, Western</term>
<term>Creutzfeldt-Jakob Syndrome (cerebrospinal fluid)</term>
<term>Creutzfeldt-Jakob Syndrome (diagnosis)</term>
<term>Creutzfeldt-Jakob Syndrome (metabolism)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Laboratories</term>
<term>Preservation, Biological</term>
<term>Protein Isoforms (metabolism)</term>
<term>Reference Standards</term>
<term>Reproducibility of Results</term>
<term>S100 Proteins (metabolism)</term>
<term>Sensitivity and Specificity</term>
<term>tau Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Conservation biologique</term>
<term>Humains</term>
<term>Isoformes de protéines (métabolisme)</term>
<term>Laboratoires</term>
<term>Maladie de Creutzfeldt-Jakob ()</term>
<term>Maladie de Creutzfeldt-Jakob (diagnostic)</term>
<term>Maladie de Creutzfeldt-Jakob (métabolisme)</term>
<term>Marqueurs biologiques (métabolisme)</term>
<term>Normes de référence</term>
<term>Protéines 14-3-3 ()</term>
<term>Protéines 14-3-3 (métabolisme)</term>
<term>Protéines S100 (métabolisme)</term>
<term>Protéines tau (métabolisme)</term>
<term>Reproductibilité des résultats</term>
<term>Sensibilité et spécificité</term>
<term>Technique de Western</term>
<term>Test ELISA</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="cerebrospinal fluid" xml:lang="en"><term>14-3-3 Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>14-3-3 Proteins</term>
<term>Biomarkers</term>
<term>Protein Isoforms</term>
<term>S100 Proteins</term>
<term>tau Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="cerebrospinal fluid" xml:lang="en"><term>Creutzfeldt-Jakob Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Creutzfeldt-Jakob Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Maladie de Creutzfeldt-Jakob</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Creutzfeldt-Jakob Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Isoformes de protéines</term>
<term>Maladie de Creutzfeldt-Jakob</term>
<term>Marqueurs biologiques</term>
<term>Protéines 14-3-3</term>
<term>Protéines S100</term>
<term>Protéines tau</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Laboratories</term>
<term>Preservation, Biological</term>
<term>Reference Standards</term>
<term>Reproducibility of Results</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Conservation biologique</term>
<term>Humains</term>
<term>Laboratoires</term>
<term>Maladie de Creutzfeldt-Jakob</term>
<term>Normes de référence</term>
<term>Protéines 14-3-3</term>
<term>Reproductibilité des résultats</term>
<term>Sensibilité et spécificité</term>
<term>Technique de Western</term>
<term>Test ELISA</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">At present, the testing of 14-3-3 protein in cerebrospinal fluid (CSF) is a standard biomarker test in suspected sporadic Creutzfeldt-Jakob disease (sCJD) diagnosis. Increasing 14-3-3 test referrals in CJD reference laboratories in the last years have led to an urgent need to improve established 14-3-3 test methods. The main result of our study was the validation of a commercially available 14-3-3 ELISA next to the commonly used Western blot method as a high-throughput screening test. Hereby, 14-3-3 protein expression was quantitatively analyzed in CSF of 231 sCJD and 2035 control patients. We obtained excellent sensitivity/specificity values of 88 and 96% that are comparable to the established Western blot method. Since standard protocols and preanalytical sample handling have become more important in routine diagnostic, we investigated in a further step the reproducibility and stability of 14-3-3 as a biomarker for human prion diseases. Ring trial data from 2009 to 2013 revealed an increase of Fleiss' kappa from 0.51 to 0.68 indicating an improving reliability of 14-3-3 protein detection. The stability of 14-3-3 protein under short-term and long-term storage conditions at various temperatures and after repeated freezing/thawing cycles was confirmed. Contamination of CSF samples with blood appears likely to be an important factor at a concentration of more than 2500 erythrocytes/μL. Hemolysis of erythrocytes with significant release of 14-3-3 protein started after 2 days at room temperature. We first define clear standards for the sample handling, short- and long-term storage of CSF samples as well as the handling of blood- contaminated samples which may result in artificially elevated CSF levels of 14-3-3.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
<li>Australie</li>
<li>Corée du Sud</li>
<li>Espagne</li>
<li>France</li>
<li>Grèce</li>
<li>Italie</li>
<li>Japon</li>
<li>Norvège</li>
<li>Pologne</li>
<li>Portugal</li>
<li>Royaume-Uni</li>
<li>Slovaquie</li>
<li>Suède</li>
</country>
<region><li>Basse-Saxe</li>
<li>Catalogne</li>
<li>Communauté de Madrid</li>
<li>Latium</li>
<li>Victoria (État)</li>
</region>
<settlement><li>Barcelone</li>
<li>Göttingen</li>
<li>Madrid</li>
<li>Melbourne</li>
<li>Rome</li>
</settlement>
<orgName><li>Université de Melbourne</li>
</orgName>
</list>
<tree><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Schmitz, Matthias" sort="Schmitz, Matthias" uniqKey="Schmitz M" first="Matthias" last="Schmitz">Matthias Schmitz</name>
</region>
<name sortKey="Ebert, Elisabeth" sort="Ebert, Elisabeth" uniqKey="Ebert E" first="Elisabeth" last="Ebert">Elisabeth Ebert</name>
<name sortKey="Karch, Andre" sort="Karch, Andre" uniqKey="Karch A" first="André" last="Karch">André Karch</name>
<name sortKey="Llorens, Franc" sort="Llorens, Franc" uniqKey="Llorens F" first="Franc" last="Llorens">Franc Llorens</name>
<name sortKey="Stoeck, Katharina" sort="Stoeck, Katharina" uniqKey="Stoeck K" first="Katharina" last="Stoeck">Katharina Stoeck</name>
<name sortKey="Zerr, Inga" sort="Zerr, Inga" uniqKey="Zerr I" first="Inga" last="Zerr">Inga Zerr</name>
</country>
<country name="Australie"><region name="Victoria (État)"><name sortKey="Collins, Steven" sort="Collins, Steven" uniqKey="Collins S" first="Steven" last="Collins">Steven Collins</name>
</region>
</country>
<country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Calero, Miguel" sort="Calero, Miguel" uniqKey="Calero M" first="Miguel" last="Calero">Miguel Calero</name>
</region>
<name sortKey="Sanchez Valle, Raquel" sort="Sanchez Valle, Raquel" uniqKey="Sanchez Valle R" first="Raquel" last="Sanchez-Valle">Raquel Sanchez-Valle</name>
</country>
<country name="Grèce"><noRegion><name sortKey="Sklaviadis, Theodor" sort="Sklaviadis, Theodor" uniqKey="Sklaviadis T" first="Theodor" last="Sklaviadis">Theodor Sklaviadis</name>
</noRegion>
</country>
<country name="France"><noRegion><name sortKey="Laplanche, Jean Louis" sort="Laplanche, Jean Louis" uniqKey="Laplanche J" first="Jean-Louis" last="Laplanche">Jean-Louis Laplanche</name>
</noRegion>
</country>
<country name="Pologne"><noRegion><name sortKey="Golanska, Ewa" sort="Golanska, Ewa" uniqKey="Golanska E" first="Ewa" last="Golanska">Ewa Golanska</name>
</noRegion>
</country>
<country name="Portugal"><noRegion><name sortKey="Baldeiras, Ines" sort="Baldeiras, Ines" uniqKey="Baldeiras I" first="Ines" last="Baldeiras">Ines Baldeiras</name>
</noRegion>
</country>
<country name="Japon"><noRegion><name sortKey="Satoh, Katsuya" sort="Satoh, Katsuya" uniqKey="Satoh K" first="Katsuya" last="Satoh">Katsuya Satoh</name>
</noRegion>
</country>
<country name="Italie"><region name="Latium"><name sortKey="Ladogana, Anna" sort="Ladogana, Anna" uniqKey="Ladogana A" first="Anna" last="Ladogana">Anna Ladogana</name>
</region>
</country>
<country name="Norvège"><noRegion><name sortKey="Skinningsrud, Anders" sort="Skinningsrud, Anders" uniqKey="Skinningsrud A" first="Anders" last="Skinningsrud">Anders Skinningsrud</name>
</noRegion>
</country>
<country name="Suède"><noRegion><name sortKey="Hammarin, Anna Lena" sort="Hammarin, Anna Lena" uniqKey="Hammarin A" first="Anna-Lena" last="Hammarin">Anna-Lena Hammarin</name>
</noRegion>
</country>
<country name="Slovaquie"><noRegion><name sortKey="Mitrova, Eva" sort="Mitrova, Eva" uniqKey="Mitrova E" first="Eva" last="Mitrova">Eva Mitrova</name>
</noRegion>
</country>
<country name="Corée du Sud"><noRegion><name sortKey="Kim, Yong Sun" sort="Kim, Yong Sun" uniqKey="Kim Y" first="Yong Sun" last="Kim">Yong Sun Kim</name>
</noRegion>
</country>
<country name="Royaume-Uni"><noRegion><name sortKey="Green, Alison" sort="Green, Alison" uniqKey="Green A" first="Alison" last="Green">Alison Green</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

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Validation of 14-3-3 Protein as a Marker in Sporadic Creutzfeldt-Jakob Disease Diagnostic.

Validation of 14-3-3 Protein as a Marker in Sporadic Creutzfeldt-Jakob Disease Diagnostic.

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